Effect of acupuncture on early onset of SSRIs treating depressive disorder and related indicators of neuroimmunology / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the intervention effect of acupuncture on early onset of selec- tive serotonin reuptake inhibitors (SSRIs) in treating depressive disorder, and to study its effect on ser- um 5-HT and unbalanced inflammatory cytokines secreted by TH1/TH2.</p><p><b>METHODS</b>Totally 90 patients with depressive disorder were randomly assigned to the drug control group (as the control group, 45 cases) and the acupuncture combined drug treatment group (as the treatment group, 45 cases). All patients were treated for 4 consecutive weeks. Another 45 healthy subjects were recruited as a healthy control group. The effect of acupuncture on early onset of SSRls in treating acute phase depressive disorder pa- tients was evaluated by HAMD score in the control group and the treatment group before treatment,and at weekends of the 1st, 2nd, and 4th week after treatment. Besides, their serum levels of 5-HT, IL-1β and IL-6 (secreted by TH1), and IL-4 and IL-10 (secreted by TH2) were detected before treatment and after treatment at the weekend of the 4th week.</p><p><b>RESULTS</b>Compared with the healthy control group,serum lev- els of 5-HT, IL-4, and IL-10 decreased in the two drug-treated groups before treatment (P < 0.01); serum levels of IL-1β and IL-6 increased (P <0.01). Compared with before treatment in the same group, HAMD score decreased in the control group at weekends of the 2nd and the 4th week after treatment (P < 0.01); HAMD scores decreased in the treatment group at weekends of the 1st, 2nd, 3rd,and 4th week after treatment (P < 0.01); serum levels of 5-HT, IL-4, and IL-10 increased,serum levels of IL-1β and IL- 6 decreased in the two drug-treated groups after treatment (all P < 0.01). Compared with the control group at the same time point,HAMD scores decreased in the treatment group at weekends of the 1st, 2nd,3rd,and 4th week after treatment (P < 0.01),serum levels of 5-HT, IL-4, and IL-10 increased (P < 0.05, P < 0.01), serum levels of IL-6 decreased (P < 0. 01).</p><p><b>CONCLUSION</b>Acupuncture could accelerate early onset of SSRIs in treating acute phase depressive disorder, and effectively regulate serum 5-HT levels and inflammatory cytokines secreted by TH1/TH2.</p>

Study of genotoxicity and oxidative stress induced by copper oxide nanoparticles in mice / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the genotoxicity and oxidative stress induced by copper oxide nanoparticles in mice.</p><p><b>METHODS</b>Thirty mice were randomly divided into control group and low- and high-dose exposure groups. The low- and high-dose exposure groups were given copper oxide nanoparticles (50 and 150 mg/kg) by a single intraperitoneal injection, while the control group was given an equal volume of normal saline containing 0.05%Tween 80. The micronucleus rate of reticulocytes in peripheral blood from the caudal vein and urinary 8-hydroxy-deoxyguanosine (8-OH-dG) level were measured before and at 24, 48, and 72 h after exposure. All the mice were sacrificed at 72 h after exposure, the liver, kidney, and femoral marrow were taken for DNA extraction, and 8-OH-dG in DNA was quantified.</p><p><b>RESULTS</b>The micronucleus rates of peripheral blood reticulocytes in low-dose exposure group at 48 h (3.11±1.46‰ and in high-dose exposure group at 24 and 48 h (4.25±0.43) and 5.42±0.76‰) were significantly increased compared with those before exposure (1.55±0.39‰ and 1.11±0.19‰) and those in control group (1.55±0.28‰ and 1.00±0.67‰) (P < 0.05 or P < 0.01). The urinary 8-OH-dG levels (ng/mg creatinine) in low- and high-dose exposure groups at all time points were significantly increased compared with those before exposure and those in control group (P < 0.05 or P < 0.01). The low- and high-dose exposure groups had significantly higher content of 8-OH-dG in liver DNA than the control group (4.53±1.27 and 7.69±2.78 vs 0.85±0.14, P < 0.01).</p><p><b>CONCLUSION</b>Copper oxide nanoparticles cause genotoxicity and increase oxidative stress in mice.</p>

Expressions of collagen in lung of rats exposed to ammonium perchlorate / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the influence on expression of interstitial collagen in lung of rats exposed to ammonium perchlorate.</p><p><b>METHODS</b>The rats were treated with AP by intratracheal instillation and sacrificed after 3 d, 7 d, 14 d, 28 d. The mRNA level of collagen I and collagen III in the lung tissues was measured by RT-PCR.</p><p><b>RESULTS</b>The levels of collagen I on 7 d, 14 d, 28 d exposed for high dose group (1.93 +/- 0.41, 3.50 +/- 0.90, 2.33 +/- 1.12) and 28 d exposed for medial dose group (2.58 +/- 0.86) were higher significant (P < 0.05) than those in negative control group (0.52 +/- 0.11, 0.77 +/- 0.15, 0.86 +/- 0.29) The levels of collagen I in low dose group exposed for 14 d(1.99 +/- 0.67), 28 d(1.85 +/- 0.67) and high dose group 14 d(3.50 +/- 0.90) exposed for 14 d were higher significant (P < 0.05) compared to those exposed to AP for 3 d(0.52 +/- 0.14), (1.71 +/- 0.38). The levels of collagen III on 14 d, 28 d exposed for high dose group (2.60 +/- 1.00, 1.46 +/- 0.36) and 14 d, 28 d exposed for medial dose group (1.80 +/- 0.51, 2.16 +/- 0.87) were higher significant (P < 0.05 or P < 0.01) than those in negative control group(0.54 +/- 0.20, 0.52 +/- 0.22); The levels of collagen III in medial dose group(2.16 +/- 0.87) exposed for 28 d, and high dose group exposed for 14 d (2.60 +/- 1.00) were higher significant (P < 0.05) compared to those exposed to AP for 3 d(1.22 +/- 0.32, 0.96 +/- 0.17).</p><p><b>CONCLUSION</b>The results suggest that AP has a toxic effect to promote the expressions of collagen I and collagen III mRNA in lungs of rats, and may be cause fibrosis, but there should have more suffice evidences to prove that AP is exactly compound that made lung fibrosis.</p>